个人简介：

闫凯歌，2011年毕业于南开大学物理科学学院，取得学士学位。同年加入清华大学高宁教授和雷建林教授课题组攻读博士，于2016年获得生物学博士学位。之后加入英国剑桥MRC分子生物学实验室英国皇家学会院士David Barford课题组从事博士后研究。自2010年起，闫博士一直从事结构生物学领域的研究，多次以第一作者在Nature, Nature Structural & Molecular Biology, Nucleic Acids Research等国际顶级期刊发表重要研究成果。2020年10月加入南方科技大学生物系。

研究领域：

本实验室综合运用冷冻电镜三维重构、生物化学及分子细胞生物学等手段，致力于解析代谢相关大分子机器的高分辨率结构及其动态调控机制。主要研究方向包括：

1. 糖原稳态的精密调控机制 — 聚焦糖原磷酸化酶激酶（PhK）和糖原脱支酶（GDE）等大型复合物，解析其组装模式与动态激活机理，阐明细胞维持能量代谢平衡的分子基础。

2. 肿瘤微环境中的氨基酸转运与靶向干预 — 揭示溶质转运蛋白（如SLC6A6/TauT）的底物识别与门控机制，并基于此设计小分子抑制剂，探索重塑肿瘤免疫微环境的治疗策略。

工作经历：

2020至今 南方科技大学生物系 助理教授

2016-2020 英国剑桥MRC分子生物学实验室 博士后

学习经历：

2011-2016 清华大学 生命科学学院 博士

2007-2011 南开大学 物理科学学院 学士

代表文章：

1. Ma R*, Du B*, Shi C*, Wang L, Zeng F, Han J, Guan H, Wang Y#, Yan K#. Molecular basis for the regulation of human phosphorylase kinase by phosphorylation and Ca²⁺. Nature Communications. 2025;16(1):3020. (#co-corresponding author; *co-first author)

2. Guan H*, Chen H*, Geng H*, Ma R, Liu Z, Wang Y, Chen Y#, Yan K#. Molecular architecture and catalytic mechanism of human glycogen debranching enzyme. Nature Communications. 2025;16(1):5962.

3. Du B*, Cheng L*, Xie J*, Chen L#, Yan K#. Molecular basis of human taurine transporter uptake and inhibition. Nature Communications. 2025;16(1):7394.

4. Liu Y*, Zhang Z*, Hu H, Chen W, Zhang F, Wang Q, Wang C, Yan K#, Du J#. Molecular basis of chromatin remodelling by DDM1 involved in plant DNA methylation. Nature Plants. 2024;10(3):374–380.

5. Xin J*, Zhou Y*, Qiu Y*, Geng H, Wang Y, Song Y#, Liang J#, Yan K#. Structural insights into AtABCG25, an angiosperm-specific abscisic acid exporter. Plant Communications. 2024;5(1):100776.

6. Ma T*, Wang L*, Chai A*, Liu C, Cui W, Yuan S, Au SWN, Sun L, Zhang X, Zhang Z, Lu J, Gao Y, Wang P, Li Z, Liang Y, Vogel H#, Wang YT#, Wang D#, Yan K#, Zhang H#. Cryo-EM structures of ClC-2 chloride channel reveal the blocking mechanism of its specific inhibitor AK-42. Nature Communications. 2023;14(1):3424.

7. Zhang H, Wang S, Zhang Z, Hou M, Du C, Zhao Z, Vogel H, Li Z, Yan K, Zhang X, Lu J, Liang Y, Yuan S, Wang D#, Zhang H#. Cryo-EM structure of human heptameric pannexin 2 channel. Nature Communications. 2023;14(1):1118.

8. Du X*, Yang Z*, Xie G, Wang C, Zhang L, Yan K, Yang M, Li S, Zhu JK, Du J#. Molecular basis of the plant ROS1-mediated active DNA demethylation. Nature Plants. 2023;9(2):271-279.

9. Niu F*, Liu Y*, Sun K, Xu S, Dong J, Yu C, Yan K, Wei Z#. Autoinhibition and activation mechanisms revealed by the triangular-shaped structure of myosin Va. Science Advances. 2022;8(49):4187.

10. Yan K*, Yang J*, Zhang Z*, McLaughlin SH, Chang L, Fasci D, Ehrenhofer-Murray AE, Heck AJR, Barford D#. Structure of the inner kinetochore CCAN complex assembled onto a centromeric nucleosome. Nature. 2019;574(7777):278–282.

11. Yan K*, Zhang Z*, Yang J, McLaughlin SH, Barford D#. Architecture of the CBF3–centromere complex of the budding yeast kinetochore. Nature Structural & Molecular Biology. 2018;25(12):1103–1110.

12. Zhang D*, Yan K*, Liu G, Song G, Luo J, Shi Y, Cheng E, Wu S, Jiang T, Lou J, Gao N#, Qin Y#. EF4 disengages the peptidyl-tRNA CCA end and facilitates back-translocation on the 70S ribosome. Nature Structural & Molecular Biology. 2016;23(2):125–131.

13. Zhang D*, Yan K*, Zhang Y*, Liu G, Cao X, Song G, Xie Q#, Gao N#, Qin Y#. New insights into the enzymatic role of EF-G in ribosome recycling. Nucleic Acids Research. 2015;43(21):10525–10533.

14. Zhang X*, Yan K*, Zhang Y, Li N, Ma C, Li Z, Zhang Y, Feng B, Liu J, Sun Y, Xu Y, Lei J#, Gao N#. Structural insights into the function of a unique tandem GTPase EngA in bacterial ribosome assembly. Nucleic Acids Research. 2014;42(21):13430–13439.

15. Gu J*, Wu M*, Guo R*, Yan K, Lei J, Gao N#, Yang M#. The architecture of the mammalian respirasome. Nature. 2016;537(7622):639–643.

16. Wu S*, Tutuncuoglu B, Yan K, Brown H, Zhang Y, Tan D, Gamalinda M, Yuan Y, Li Z, Jakovljevic J, Ma C, Lei J, Dong MQ, Woolford JL Jr#, Gao N#. Diverse roles of assembly factors revealed by structures of late nuclear pre-60S ribosomes. Nature. 2016;534(7605):133–137.

17. Yatskevich S*, Kroonen JS, Alfieri C, Tischer T, Howes AC, Clijsters L, Yang J, Zhang Z, Yan K, Vertegaal ACO, Barford D#. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Reports. 2021;34(13):108929.

18. Wang W*, Li W*, Ge X*, Yan K, Mandava CS, Sanyal S#, Gao N#. Loss of a single methylation in 23S rRNA delays 50S assembly at multiple late stages and impairs translation initiation and elongation. Proc Natl Acad Sci U S A. 2020;117(27):15609-15619.

19. Ma C*, Wu S, Li N, Chen Y, Yan K, Li Z, Zheng L, Lei J, Woolford JL Jr, Gao N. Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. Nature Structural & Molecular Biology. 2017;24(3):214-220.

20. Ma C*, Yan K, Tan D, Li N, Zhang Y, Yuan Y, Li Z, Dong MQ, Lei J, Gao N. Structural dynamics of the yeast Shwachman-Diamond syndrome protein (Sdo1) on the ribosome and its implication in the 60S subunit maturation. Protein Cell. 2016;7(3):187-200.

21. Zhang J*, Pan X, Yan K, Sun S, Gao N#, Sui SF#. Mechanisms of ribosome stalling by SecM at multiple elongation steps. Elife. 2015;4: e09684.

22. Yang Z*, Guo Q*, Goto S, Chen Y, Li N, Yan K, Zhang Y, Muto A, Deng H, Himeno H, Lei J#, Gao N#. Structural insights into the assembly of the 30S ribosomal subunit in vivo: functional role of S5 and location of the 17S rRNA precursor sequence. Protein Cell. 2014;5(5):394-407.